Assisted Hatching and Zona Dissection Micropipettes

Assisted Hatching and Zona Dissection Micropipettes 3
Assisted Hatching and Zona Dissection Micropipettes 1

A comprehensive range for all preferences

Assisted hatching micropipettes are used to direct acidic solution onto the zona pellucida to thin the zona or create a hole to enable the embryo to hatch.

Zona dissection micropipettes are used to mechanically cut an opening in the zona pellucida of an oocyte or embryo to enable assisted hatching and biopsy.

With two ranges of pipettes, we offer a wide variety of sizes and shapes so you can choose what best fits your requirements.

Availability

These products are available for sale in selected countries worldwide.

Assisted Hatching Micropipettes

The outer protein membrane that surrounds the embryo or blastocyst is weakened to facilitate hatching and the eventual implantation of the embryo. These micropipettes can be used to create zona opening before biopsy or ICSI, and additionally for collapsing blastocysts before vitrification.

CooperSurgical® assisted hatching micropipettes are crafted with precision and innovation; they offer control in creating an opening in the zona pellucida.

ORIGIO® Assisted Hatching Micropipettes

  • Assisted Hatching micropipettes have a flat, slightly polished opening
  • Two inner diameter sizes are available: 8 or 10µm

The Pipette Company TPC™ Assisted Hatching Micropipette

  • Flat, square and lightly heat-polished to reduce the possibility of damage to the embryo or polar body due to sharp edges

If you choose not to use laser-drilling of the zona pellucida, we recommend using the assisted hatching pipette (MAH-SM-(angle)) in combination with acidic solution to create a hole in the zona.

A smaller ID provides better control of the acidic solution.

CooperSurgical recommends the use of a laser. Laser-assisted hatching is a standardized method.
To compare with the use of laser, please refer to the information about our RI Saturn 5TM Laser System.

Partial Zona Dissection (PZD) Micropipettes

PZD micropipettes play a role in both biopsy and mechanical hatching techniques by serving as precise tools to physically penetrate the protective barrier known as the zona pellucida. Our micropipettes are designed to make controlled incisions or punctures in the zona pellucida, enabling targeted access to the embryo.

These micropipettes are fine and precise instruments, often with a tapered or beveled tip, allowing for controlled cutting or thinning of the zona pellucida without causing significant damage to the embryo.

ORIGIO® PZD Micropipettes

  • The sharp tip of the micropipette mechanically creates a breach in the zona pellucida around the oocyte or embryo
  • PZD micropipette is designed with a long thin taper to a sharp point
  • Available angle options: 30 or 35 degrees

TPC™ PZD Micropipettes

  • Fine tip and a sharp cutting edge
  • Ergonomic design to support manual and micromanipulation handling

A sharper tip makes it easier to penetrate the zona pellucida as it is a non-elastic structure.

Packaging

Assisted Hatching and Zona Dissection Micropipettes 5

ORIGIO pipettes packaging

All our ORIGIO micropipettes are packaged in a transparent compact box of 10.

  • Utilizes less shelf space
  • Allows for a very quick overview of how many pipettes are left
  • Includes tip protector in each micropipette tube

Please watch the video if you would like to see how easy it is to take an ORIGIO micropipette out of its packaging.

Assisted Hatching and Zona Dissection Micropipettes 6

TPC pipettes packaging

All our TPC micropipettes are packaged in a recyclable cardboard box of 10. CooperSurgical encourages all clinics to recycle when possible.

Pipettes are individually packaged and gamma sterilized for optimum sterility. For easy identification, pipettes are packed in color-coded protective holders. Outer labels are also color-coded.

Reference:

  1. Nagy, Z.P., Varghese, A.C. and Agarwal, A. eds., 2019. In vitro fertilization: a textbook of current and emerging methods and devices, section 47.7 Mechanical Opening

Product codes

ORIGIO® Assisted Hatching Micropipettes (10 per box)

ReferencePipette TypeTip AngleInternal Diameter Distance tip to bend
MAH-LG-30Hatching 30º 10-12µm0.5mm
MAH-LG-35 Hatching 35º 10-12µm0.5mm

TPCAssisted Hatching Micropipettes (10 per box)

ReferencePipette TypeTip AngleInternal Diameter Distance tip to bend
LDSZona Drilling30º9 µm1mm

ORIGIO® Zona Dissection Micropipettes (10 per box)

ReferencePipette TypeTip AngleDistance tip to bend
PZD-30PZD Micropipet 30º 0.5mm
PZD-35PZD Micropipet 35º 0.5mm

TPC™ Zona Dissection Micropipettes (10 per box)

ReferencePipette TypeTip AngleDistance tip to bend
LCSZona Cutting30º1mm

Get In Touch With Us

We’d love to hear from you. How can we help?

If you choose not to use laser-drilling of the zona pellucida, we recommend using the Assisted Hatching pipet (MAH-SM-(angle)) in combination with the Acidified Tyrodes media to create a hole in the zona. This means on the one side of the micromanipulator you need a double pipet holder to have the Assisted Hatching and the biopsy pipet both “in play.”

Procedures

Procedure-culture-FI

Culture

Creating the optimal environment for human embryology procedures

Procedure-embryoBiopsy-FI

Embryo Biopsy

The full solution for efficient and effective biopsy procedures

Want unlimited First line support

All our USA and Europe Customers get free unlimited first line support with a service contract.

Support & Compliance

Our global team is committed to providing the highest standards of service and support.

Assisted Hatching and Zona Dissection Micropipettes 9

Batch Certificates

Use this tool to enter your batch number and download the corresponding certificate of analysis.

Assisted Hatching and Zona Dissection Micropipettes 10

Service

We offer a range of contract options to suit your needs: preventative maintenance and service, reliable access to spare parts, product training, and online handling of service requests.

Assisted Hatching and Zona Dissection Micropipettes 11

Hardware Warranty

Submit your Product Warranty Card online by completing our quick and easy form.

Get In Touch With Us

We’d love to hear from you. How can we help?