SAGE 1-Step™ Medium for Uninterrupted Embryo Culture
A refined, robust single-step medium with hyaluronan for embryologists worldwide
SAGE 1-Step™ medium with hyaluronan provides an environment to support continuous embryo culture from zygote to blastocyst formation, through to transfer and utilization.3,4
Designed with the human embryo
in mind
Hyaluronan is abundant in the female reproductive tract and has a demonstrated role in embryo development and implantation.1,2
The combination of hyaluronan and human serum albumin (HSA) in culture medium has been shown to significantly increase embryo development.2,3
Refined and Robust
Designed with an optimized combination of nutrients, amino acids, antioxidants, and macromolecules,3,6,7 SAGE 1-Step is formulated with energy substrates that support the changing nutritional needs of the developing embryo.3,6
- Formulation aids embryonic control of internal pH and provides a visual indicator of media pH.3,5,6
- Increased levels of antioxidants provide a protective effect during embryo culture.7
- The inclusion of essential and non-essential amino acids is associated with improved blastocyst formation rates and viability.6,8
Your Partner in Culture
CooperSurgical is dedicated to upholding the highest standards, providing reliable solutions and expert support to optimize your lab’s daily outcomes. Our expert clinical support teams are available to assist you with workflow reviews, set up media evaluations, and provide in-clinic training. Get exclusive access to our clinical support and professional education teams.
Key Specifications
Recommended pH: 7.3 ± 0.1
HSA: 5 mg/mL
Osmolality: 257–273 mOsm/kg
Mouse Embryo Assay (MEA): >80% (BL Rate/1-cell)
Shelf Life: 26 weeks from manufacture; 7 days from opening
Compatibility
SAGE 1-Step™ integrates seamlessly into your existing lab ecosystem. For a fully optimized culture system, discover CooperSurgical’s range of quality oils, GPS™ dishes, incubators, and fertilization media.
Discover our integrated culture solutions
Product codes
Available in convenient 10mL and 60mL bottles to suit your specific laboratory requirements.
SAGE 1-Step HSA is available for sale in Europe, the U.S.A, Canada and Australia as well as selected countries in the rest of the world.
| Product | Order Code | Size | HSA mg/mL | SPS mg/mL | Shelf Life Guarantee* |
|---|---|---|---|---|---|
| SAGE 1-Step™ with HSA and phenol red | 67010010 | 10mL | 5 mg/mL | – | Minimum 8 weeks |
| SAGE 1-Step™ with HSA and phenol red | 67010060 | 60mL | 5 mg/mL | – | Minimum 8 weeks |
Brochures, Catalogs & Flyers
Product Data Sheet
Product Data Sheet
Optimizing Culture Brochure
Optimizing Culture Brochure
Instructions for Use
Instructions for Use
Other Information
- Magnesium sulphate
- Potassium chloride
- Potassium phosphate
- Sodium chloride
- Amino acids
- L-alanyl-L-glutamine
- Calcium-L-lactate
- Glucose · Sodium pyruvate
- Sodium bicarbonate
- EDTA · Gentamicin
- Phenol red
SAGE 1-Step™ with HSA (6701) only:
- Sodium hyaluronate
- Human Serum Albumin
SAGE 1-Step™ with SPS (6702) only:
- Serum Protein Supplement (SPS)
- CE marked (CE 0543): SAGE 1-Step with HSA only
- Cleared by the FDA
- Canada Medical Device licensed
- Sterility tested (Ph. Eur., USP)
- Osmolality tested (Ph. Eur., USP)
- pH tested (Ph. Eur., USP)
- Endotoxin tested ≤ 0.15 EU/ml (Ph. Eur., USP)
- Mouse Embryo Assay (MEA)
Take the next step
Ready to optimize your culture system?
Get in touch with your local CooperSurgical representative to learn more about how
SAGE 1-Step can optimize your embryo culture workflows.
References
- Bontekoe, S., Johnson, N. and Blake, D. (2014) Adherence compounds in embryo transfer media for assisted reproductive technologies. Cochrane Database Systematic Reviews, Issue 2. Art.No.:CD007421.
- Fouladi‑Nashta, A.A., et al. (2017) Regulation and roles of the hyaluronan system in mammalian reproduction. Reproduction, Feb; 153, pp. R43‑R58.
- Lane, M. and Gardner, D.K. (2007) Embryo culture medium: which is the best? Best Practice & Research Clinical Obstetrics and Gynecology. Vol. 21, No. 1, pp. 83e100.
- Gruber, I., and Klein, M. (2011) Embryo Culture media for human IVF: which possibilities exist? J Turk Ger Gynecol Assoc.1;12(2):110–117.
- Blake, D., et al. (2002) Protein supplementation of human IVF culture media. J Assist Reprod Genet. Mar;19(3):137‑43.
- Quinn, P. (2012) Culture Systems: Sequential Embryo Culture: Methods and Protocols. Methods in Molecular Biology. Vol. 912, pp: 211‑230.
- Agarwal, A., et al. (2014) Utility of antioxidants during assisted reproductive techniques: an evidence‑based review. Reprod Biol Endocrinol. 12:112.
- Kobanawa, M. (2022) Fertilization, embryo culture, and clinical results using low lactate embryo culture medium for pre‑culture, insemination, and beyond. Reprod Med Biol, 21(1), e12458; pp.1‑9.
Get In Touch With Us
We’d love to hear from you. How can we help?