ORIGIO® Micropipettes
Trusted micropipettes for micromanipulation procedures
The range of ORIGIO micropipettes is extensive, catering to various stages of the IVF cycle. Each micropipette is specifically crafted for clinical applications, spanning all micromanipulation procedures and is meticulously designed to meet the standards of clinical use, helping to ensure precision and reliability in tasks such as intracytoplasmic sperm injection (ICSI), embryo biopsy, and assisted hatching.
- First to market commercially! Quality pipettes available since 1992
- More than 1.5 million micropipettes manufactured each year
- Automated production – focus on innovation, consistency and deliverability
- Every pipette is checked and measured repeatedly during manufacture
ORIGIO micropipettes are designed to be used in conjunction with a micromanipulator syringe, within the micromanipulation equipment system, and with a microscope.
Availability
These products are available for sale in Europe, the USA, Canada and Australia, as well as selected countries in the rest of the world.
Did you know?
In 1992 the first commercial high-quality micropipettes for IVF professionals were marketed by ORIGIO, previously Humagen.
Quality and consistency
Since 1992, CooperSurgical has manufactured consistently high-quality micropipettes for your IVF clinic. At our manufacturing facility we have a focus on quality assurance at every step.
- 100% visual inspection for dimensional tolerances and cleanliness at all steps of production
- Cleaning, heating, and sonication of borosilicate glass capillaries
- Our micropipettes are produced in a cleanroom environment
- Regular bioburden testing of the cleanroom
- Air filters to remove VOCs, bacteria, and particles from the air in the manufacturing cleanroom
- Operators wear cleanroom apparel
- Latest technology in manufacturing lab
Sterilization and IVF quality control methods
CooperSurgical performs rigorous quality control measures to ensure the safety and efficacy of the micropipettes used in IVF.
- All our micropipettes are sterilized by gamma irradiation to a Sterilization Assurance Level (SAL) of 10^-6
- A sample of each batch is sent for Mouse Embryo Assay (MEA) testing and Limulus amoebocyte lysate (LAL) toxicological bioassay to detect toxicity and suboptimal compounds for embryo development
- A MEA is conducted in accordance with FDA Industry Guidance. (8) >80% 1-cell blastocyst development rate in 96 hours. LAL <20 Endotoxin Units (EU)/device Batch certificates are available online which enables easier record keeping for quality and regulatory assurance
Packaging
All our ORIGIO micropipettes are packaged in a transparent compact box of 10.
- Minimizes the space taken up on your shelves
- Allows for a very quick overview of how many pipettes are left
- Tip protector included in each micropipette tube
Please watch the video if you would like to see how easy it is to take an ORIGIO micropipette out of its packaging.
ORIGIO® ICSI Micropipettes
Our extensive range of ICSI micropipettes is versatile and precisely designed to inject sperm into oocytes. We offer a large range so we can meet the diverse needs and preferences of embryologists worldwide. Whether you need specific dimensions or have ergonomic requirements, our ICSI micropipettes offer a suite of options to help enhance performance in the lab.
Our customer’s favourite: ICSI SlimLine
The size of these popular pipettes is designed to help maximize normal fertilization rate.4
ICSI SlimLine pipettes are designed with a long parallel taper that some will find more flexible than our standard ICSI pipettes. When injecting they keep the rigidity that users like from the ORIGIO micropipettes.
“The design of the new ICSI Slimline pipet allows for an extremely gentle injection procedure, which practically eliminates oocyte damage.”
Marlena Duke, M.Sc.,
Embryology Laboratory Director, RMA of New York
The ORIGIO SlimLine ICSI micropipette is designed with a small inner diameter and a longer parallel taper to:
- Provide enhanced fluid control1, 2
- Maximize normal fertilization rate4
- Minimize postinjection degeneration2
- Reduction in tripronuclear fertilization2
- Increase the proportion of good-quality embryos and higher cell numbers 2
- Help minimize damage to the oocyte by creating a small entry point
Classic ORIGIO ICSI Micropipettes
If you do prefer a pipette with a short taper, we have a full range of ICSI micropipettes available.
Key features
- Short taper when a more rigid tip is needed; some find this easier to puncture the oocyte
- Small inner diameter minimizes the likelihood of oocyte damage1, 2, 3
- Short bevel helps reduce the risk of sperm getting caught on glass surface during injection
Spike or no spike? it is your choice!
- Most ORIGIO ICSI micropipettes are designed with fine spikes for easy penetration of the zona pellucida and minimized oocyte distortion.5 Users like this feature as it gives them a feeling of a sharper micropipette.
- We also have ORIGIO ICSI micropipettes with no spike for embryologists more focused on decreasing the risk of ooplasm leakage after ICSI, and cytoplasm aspiration:3,5 MIC-NSP-0, MIC-NSP-30, MIC-NSP-35 and MICC001-30-B1.0.
Good to know
- CooperSurgical ICSI micropipettes with a large Inner Diameter (I.D.) are designed to easily pick up sperm from surgically retrieved sperm samples.6
- MIC-50-xx ICSI micropipettes are designed to minimize trauma to the oocyte during the injection process.5
- ICSI micropipettes with a flat tip can be used for Piezo-ICSI:5 MICC048-0 or MICC048-25.
There are several different types of ICSI micropipettes available, each with their unique characteristics and features. The most common types include blunt and sharp-tipped micropipettes, as well as micropipettes with a beveled or angled tip. The choice of micropipette will depend on factors such as the size and quality of the oocytes and sperm being used, the preferences of the fertility specialist or embryologist, and the specific requirements of the fertility treatment.
To optimize results, ICSI micropipettes should be used together with a high-quality microscope, a micromanipulation system and well-functioning injectors.
Using an ICSI micropipette requires skill and precision, as the technique is highly delicate and requires a well-trained hand. Regular training and practice can improve your technique and help ensure consistent results. Read more about our possibilities for training here:
What is the main difference between TPC and ORIGIO micropipettes?
TPC and ORIGIO micropipettes are designed differently. TPC ICSI micropipettes have a longer and parallel taper, while ORIGIO ICSI micropipettes are more conically shaped. TPC ICSI micropipettes will therefore feel more flexible and bendable, while ORIGIO ICSI micropipettes feel more rigid. In general, TPC ICSI micropipettes have a longer bevel and shorter spike, while ORIGIO ICSI micropipettes have a shorter bevel and a longer spike.
When it comes to the holding micropipettes, TPC has a squarer surface while ORIGIO has a more rounded shape.
In addition, all our ORIGIO micropipettes are packaged in a transparent compact box of 10 and all our TPC micropipettes are packaged in a recyclable cardboard box of 10. Both lines are individually packaged and color-coded.
- When selecting micropipettes for IVF applications, what factors do I need to consider?
When choosing micropipettes for In Vitro Fertilization (IVF), we recommend that you pay specific attention to:
- Materials: IVF-compatible materials ensure you do not introduce any contaminants or toxins into the IVF process
- Ease of handling and operation: Consider ergonomic designs that reduce the risk of operator fatigue or error
ORIGIO® Biopsy Micropipettes
Our micropipettes are designed with precision and flexibility in mind, supporting operators with the right tools for the distinct requirements with all IVF biopsy techniques.
From polar body examinations to blastomere and trophectoderm procedures, our tailored biopsy micropipettes help to empower handlers with the reliability and performance needed to excel in the intricacies of genetic testing.
- Designed to remove polar bodies or other cells for genetic analysis
- Polar body biopsy micropipette options are: flat/polished, beveled/polished, and beveled/spiked
- CooperSurgical biopsy pipettes with flat tips are designed to reduce the risk of damaging the cells or embryos during biopsy6
- Blastomere biopsy options come in five sizes and include small – medium: a range of inner diameter (20-30µm ID) allow biopsy of blastomeres and trophectoderm
Changing the biopsy pipette for each embryo/oocyte to avoid cross-contamination.7
It is the same as for any micropipette used for aspiration, such as ICSI pipettes.
All our pipettes should be stored in the original unopened packaging, away from contamination, moisture and direct heat at room temperature.
ORIGIO® Holding Micropipettes
- Large diameter ratio for enhanced control
- High-quality holding micropipettes from the first name in micropipettes
- Covering all micromanipulation needs: available in small, medium, large and extra large
ORIGIO® Assisted Hatching Micropipettes
- To mechanically create a breach in the zona pellucida around the oocyte or embryo with the sharp tip of the micropipette
- Assisted Hatching Micropipettes have a blunt, slightly polished opening
- Two Inner Diameter sizes offered: 8 or 10µm
ORIGIO® Partial Zona Dissection (PZD) Micropipettes
- To mechanically create a breach in the zona pellucida around the oocyte or embryo with the sharp tip of the micropipette
- PZD pipette is pulled with a long thin taper to a sharp point
- Available angle options: 30 or 35 degrees
References
- Bryan Woodward (2010), “Commentary: Oocyte degeneration following ICSI”, Journal of Reproductive and stem cell biotechnology, Vol 1, No 9, page 193
- Yavas, Y.A.L.C.I.N., Roberge, S.Y.L.V.I.E., Khamsi, F.I.R.O.U.Z., Shirazi, P.A.R.V.I.N., Endman, M.W. and Wong, J.C., 2001. Performing ICSI using an injection pipette with the smallest possible inner diameter and a long taper increases normal fertilization rate, decreases incidence of degeneration and tripronuclear zygotes, and enhances embryo development. Journal of assisted reproduction and genetics, 18(8), pp.426-435.
- Vanderzwalmen, P., Bertin, G., Lejeune, B., Nijs, M., Vandamme, B. and Schoysman, R., 1996. Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema. Human Reproduction, 11(3), pp.540-547.
- Terriou et al. “Comparison of ICSI results obtained using a needle with a special slim profile to reduce lysis of the oocyte (Slimline, ORIGIO).” at FFER congress 2017.
- Dianna Payne (2010), “Commentary: Oocyte degeneration following ICSI”, Journal of Reproductive and stem cell biotechnology, Vol 1, No 9, page 193
- Nagy, Z.P., Varghese, A.C. and Agarwal, A. eds., 2019. In vitro fertilization: a textbook of current and emerging methods and devices, section 31.10 Oocyte-Sperm Co-incubation: The Devices, section 25.7.1 Utility of Electronic Medical Records
- ESHRE PGT Consortium and SIG-Embryology Biopsy Working Group; Kokkali G, Coticchio G, Bronet F, Celebi C, Cimadomo D, Goossens V, Liss J, Nunes S, Sfontouris I, Vermeulen N, Zakharova E, De Rycke M. ESHRE PGT Consortium and SIG Embryology good practice recommendations for polar body and embryo biopsy for PGT. Hum Reprod Open. 2020 May 29;2020(3): hoaa020. doi: 10.1093/hropen/hoaa020. PMID: 32500104; PMCID: PMC7257009.
Product codes
ICSI Micropipettes
| Type | Order Code | Bend angles (XX in ref. no.) | Tip to bend distance | Bevel angle | Bevel length | Inner diameter(μm) | Taper type |
|---|---|---|---|---|---|---|---|
| ICSI SlimLine | MIC-SLM-XX | 0°, 20°, 25°, 30°, 35° | 0.5mm | 35-40° | 10-12.5μm | 4.3-4.9µm | Parallel Taper |
| ICSI SlimLine | MIC-SLM-XX-B1.0 | 30°, 35° | 1mm | 35-40° | 10-12.5μm | 4.3-4.9µm | Parallel Taper |
| ICSI | MIC-35-XX | 30°, 35° | 0.5mm | 30-35° | 10-12.5μm | 5.0-5.5µm | Standard Taper |
| ICSI | MIC-35-XX-B1.0 | 30°, 35° | 1mm | 30-35° | 10-12.5μm | 5.0-5.5µm | Standard Taper |
| ICSI | MIC-SI-XX | 20°, 25°, 30°, 35° | 0.5mm | 35-40° | 10-12μm | 5.0-5.5µm | Standard Taper |
| ICSI | MIC-SI-XX-B1.0 | 30°, 35° | 1mm | 35-40° | 10-12μm | 5.0-5.5µm | Standard Taper |
| ICSI | MIC-50-XX | 0°, 20°, 25°, 30°, 35° | 0.5mm | 50° | 7.5-9.5μm | 5.0-5.5µm | Standard Taper |
| ICSI | MIC-50-XX-B1.0 | 30° | 1mm | 50° | 7.5-9.5μm | 5.0-5.5µm | Standard Taper |
| Large ICSI | MIC-8-XX | 30° | 0.5mm | 50° | 7.5-9.5μm | 6.2-7.0µm | Standard Taper |
| Large ICSI | MIC-9-XX | 30°, 35° | 0.5mm | 40-45° | 9-11.5μm | 6.5-8.0µm | Standard Taper |
Holding Micropipettes
| Type | Order Code | Bend angles (XX in ref. no.) | Tip to bend distance | Inner diameter(μm) | Outer diameter(μm) |
|---|---|---|---|---|---|
| Small Holding | MPH-SM-XX | 0°, 20°, 25°, 30°, 35° | 0.4mm | 17-22µm | 77-87µm |
| Medium Holding | MPH-MED-XX | 0°, 20°, 25°, 30°, 35° | 0.4mm | 17-22µm | 100-110µm |
| Medium Holding | MPH-MED-XX-B1.0 | 30°, 35° | 1.0mm | 17-22µm | 100-110µm |
| Large Holding | MPH-LG-XX | 0°, 20°, 25°, 30°, 35° | 0.4mm | 23-28µm | 110-125µm |
| Extra Large Holding | MPH-XLG-XX | 0°, 25°, 30°, 35° | 0.4mm | 23-28µm | 130-145µm |
Biopsy Micropipettes
| Tip type | Order Code | Bend angles (XX in ref. no.) | Tip to bend distance | Bevel | Inner diameter(μm) |
|---|---|---|---|---|---|
| Flat and Polished | MBB-FP-SM-XX | 0°, 20°, 25°, 30°, 35° | 0.4mm | – | 27.5-32.5 |
| Flat and Polished | MBB-FP-M-XX | 0°, 20°, 25°, 30°, 35° | 0.4mm | – | 32.5-37.5 |
| Flat and Polished | MBB-FP-L-XX | 30°, 35° | 0.4mm | – | 37.5-42.5 |
| Flat and Polished | MBB-FP-XXS-XX | 30°, 35° | 0.4mm | – | 18-22 |
| Flat and Polished | MBB-FP-XS-XX | 30°, 35° | 0.4mm | – | 23-27 |
| Bevelled and Polished | MBB-BP-SM-XX | 30°, 35° | 0.4mm | 45° | 27.5-32.5 |
| Bevelled and Polished | MBB-BP-M-XX | 30°, 35° | 0.4mm | 45° | 32.5-37.5 |
| Small, Bevelled and Spiked | MPB-BS-XX | 30° | 0.45mm | 45° | 14-16 |
| Small, Flat and Polished | MPB-FP-XX | 30° | 0.45mm | – | 13-15 |
Hatching and Dissection
| Type | Order Code | Bend angles (XX in ref. no.) | Tip to bend distance | Inner diameter(μm) |
|---|---|---|---|---|
| Assisted Hatching | MAH-SM-XX | 30°, 35° | 0.4mm | 8-10µm |
| Zona Dissection | PZD-XX | 30°, 35° | 0.45mm | – |
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